Journal: Lasers in Medical Science

Article Title: Effects of low level laser on periodontal tissue remodeling in hPDLCs under tensile stress

doi: 10.1007/s10103-023-03885-0

Figure Lengend Snippet: Gene sequence

Article Snippet: The primary antibodies were listed in the following manner: GAPDH (ABclonal,1:5000,USA), NFATC1 (ABclonal,1:1000,USA), TIMP-1 (ABclonal,1:1000,USA),TIMP-2 (ABclonal,1:1000,USA), MMP-1 (ABclonal,1:1000,USA),MMP-2 (ABclonal,1:1000,USA), Runx2 (ABclonal,1:1000,USA),MMP-9 (ABclonal,1:1000,USA), COL-I (ABclonal,1:1000,USA), COL-III (ABclonal,1:1000,USA), c-Fos (CST, 1:1000, US), OCN (CST, 1:1000), CTSK (Abcam,1:1000).

Techniques: Sequencing

Journal: Lasers in Medical Science

Article Title: Effects of low level laser on periodontal tissue remodeling in hPDLCs under tensile stress

doi: 10.1007/s10103-023-03885-0

Figure Lengend Snippet: Collagen-modified factors detection ( a ) Quantitative real-time PCR analysis of Col-I and Col-III mRNA expression of hPDLCs. n = 3 . ( b ) Western blot analysis of Col-I and Col-III protein levels of hPDLCs. n = 3 . Data represent the mean ± SD of three independent experiments. *P < 0.05 vs. the control group, #P < 0.05 vs. the tensile stress group and &P < 0.05 vs. the laser group by one-way ANOVA

Article Snippet: The primary antibodies were listed in the following manner: GAPDH (ABclonal,1:5000,USA), NFATC1 (ABclonal,1:1000,USA), TIMP-1 (ABclonal,1:1000,USA),TIMP-2 (ABclonal,1:1000,USA), MMP-1 (ABclonal,1:1000,USA),MMP-2 (ABclonal,1:1000,USA), Runx2 (ABclonal,1:1000,USA),MMP-9 (ABclonal,1:1000,USA), COL-I (ABclonal,1:1000,USA), COL-III (ABclonal,1:1000,USA), c-Fos (CST, 1:1000, US), OCN (CST, 1:1000), CTSK (Abcam,1:1000).

Techniques: Modification, Real-time Polymerase Chain Reaction, Expressing, Western Blot, Control

Journal: Frontiers in Medicine

Article Title: The Blockade of TACE-Dependent EGF Receptor Activation by Losartan-Erlotinib Combination Attenuates Renal Fibrosis Formation in 5/6-Nephrectomized Rats Under Vitamin D Deficiency

doi: 10.3389/fmed.2020.609158

Figure Lengend Snippet: Analysis of extracellular matrix proteins expression in the renal cortex evaluated 60 days after 5/6 nephrectomy in vitamin D-deficient rats treated with losartan, erlotinib or losartan+erlotinib. (A) Quantitative amount of collagen III (Col III) evaluated by ELISA in N+D, N+D+L, N+D+E and N+D+L+E groups. (B) Bar graph of immunohistochemical analysis of fibronectin expression in N+D, N+D+L, N+D+E and N+D+L+E groups. (C) Representative photomicrographs of immunostaining for fibronectin in the renal cortex from a N+D, N+D+L, N+D+E and N+D+L+E rat (x400). Note that fibronectin expression is significantly lower in the N+D+L+E than in the other groups. a p < 0.001, b p < 0.01 vs. N+D; f p < 0.05 vs. N+D+L; h p < 0.01, i p < 0.05 vs. N+D+E. N, 5/6-nephrectomized rats; D, vitamin D-deficient rats; L, losartan-treated rats; E, erlotinib-treated rats; L+E, rats concomitantly treated with losartan+erlotinib. N+D ( n = 10); N+D+L ( n = 8); N+D+E ( n = 7); N+D+L+E ( n = 10).

Article Snippet: We assessed Collagen Type III (Col III) (#MBS2023591), TGF-β1 (#MBS824788), and EGF (#MBS824918) in renal cortex samples by ELISA using commercial kits (MyBiosource, San Diego, CA), following the instructions provided by the manufacturer.

Techniques: Expressing, Enzyme-linked Immunosorbent Assay, Immunohistochemical staining, Immunostaining